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Canine Coat and Nose Color Testing


C128-Canine Coat and Nose Color Test
Great Dane

DNA TEST

Great Danes occur in a wide variety of coat colors. Although the alleles causing all the coat colors have not yet been identified, DNA tests are available to identify the presence of some of these, which are outlined below.
There are two common alleles in Great Danes at the E locus: Em and E. Em produces a melanistic (dark) mask. It is only visible on fawn or brindle dogs. So the mask is not visible on Harlequin, Mantle, merle, or white Danes, and is not distinguishable from the body color on black and blue Danes. Since the presence of mask is dominant to its absence, one does not know if a dog with a mask is Em/E or Em/Em, unless one parent did not have mask without DNA testing.
Blue in Great Danes is caused by the presence of two d alleles. Blue is carried by some black, fawn and other colored Great Danes. A dog with only one d allele is never blue, but can produce blue. A d/d genotype would cause a fawn Great Dane to have a blue mask instead of a black mask if the Em allele is present. A d/d genotype would cause a brindle Great Dane to have blue instead of black stripes on a pale fawn.
All Great Danes are ay/ay at the agouti locus so there is no need to test for alleles at the agouti locus. Although DNA tests are not yet available for alleles of the K locus, recent research has shown that black or blue dogs have at least one K allele. At the present time there is no test to distinguish which black or blue dog might carry brindle or fawn, which is caused by the presence of recessive alleles at the K locus.
Brindle dogs have at least one copy of the kbr allele (they may have two). Fawn dogs do not carry brindle because they are k/k. There is also no DNA test for Harlequin at this time. It has recently been proven that Harlequin dogs all carry at least one copy of the merle allele.

Notes
Danes can carry one of the alleles causing brown, although these are very rare. Dogs that are b/b, will have chocolate coats instead of black or pale brown coats instead of blue. The markings would be chocolate on the Harlequin or merle dogs. Testing for the b alleles is not routine, but is available at no extra charge.

The alleles at the A and E locus were identified by a collaborative research project between the labs of Dr. Greg Barsh at Stanford University and Dr. Sheila Schmutz at the University of Sascatchewan, who also identified the B locus alleles. The d allele has been identified by a collaborative research project between the labs of Dr. Tosso Leeb and Dr. Schmutz. Many dogs owners helped by contributing either individual samples or samples from complete litters.

GENOTYPES
Please select the color of your dog to determine possible genotypes:
Genotype Main Color Nose Color Hidden Color
KbKbEmEmDDSS black black hidden mask
KbkxEmEmDDSS black black hidden mask, brindle or fawn
KbKbEmEDDSS black black hidden mask, lack of mask
KbkxEmEDDSS black black hidden mask, brindle or fawn lack of mask
KbKbEEDDSS black black none
KbkxEEDDSS black black brindle or fawn
KbKbEmEmDdSS black black hidden mask, dilute
KbkxEmEmDdSS black black hidden mask, brindle or fawn, dilute
KbKbEmEDdSS black black hidden mask, lack of mask, dilute
KbkxEmEDdSS black black hidden mask brindle or fawn lack of mask dilute
KbKbEEDdSS black black dilute
KbkxEEDdSS black black brindle or fawn, dilute
KbKbEmEmDDSsp black black hidden mask excessive white
KbkxEmEmDDSsp black black hidden mask brindle or fawn excessive white
KbKbEmEDDSsp black black hidden mask, lack of mask, excessive white
KbkxEmEDDSsp black black hidden mask, brindle or fawn, lack of mask, excessive white
KbKbEEDDSsp black black excessive white
KbkxEEDDSsp black black brindle or fawn, excessive white
KbKbEmEmDdSsp black black hidden mask, dilute, excessive white
KbkxEmEmDdSsp black black hidden mask, brindle or fawn, dilute, excessive white
KbKbEmEDdSsp black black hidden mask, lack of mask, dilute, excessive white
KbkxEmEDdSsp black black hidden mask, brindle or fawn, lack of mask, dilute, excessive white
KbKbEEDdSsp black black dilute, excessive white
KbkxEEDdSsp black black brindle or fawn, dilute, excessive white
KbKbEmEmDDspsp black with excessive white black hidden mask
KbkxEmEmDDspsp black with excessive white black hidden mask, brindle or fawn
KbKbEmEDDspsp black with excessive white black hidden mask, lack of mask
KbkxEmEDDspsp black with excessive white black hidden mask, brindle or fawn, lack of mask
KbKbEEDDspsp black with excessive white black none
KbkxEEDDspsp black with excessive white black brindle or fawn
KbKbEmEmDdspsp black with excessive white black hidden mask dilute
KbkxEmEmDdspsp black with excessive white black hidden mask, brindle or fawn, dilute
KbKbEmEDdspsp black with excessive white black hidden mask, lack of mask, dilute
KbkxEmEDdspsp black with excessive white black hidden mask, brindle or fawn, lack of mask, dilute
KbKbEEDdspsp black with excessive white black dilute
KbkxEEDdspsp black with excessive white black brindle or fawn, dilute
KbKbEmEmddSS blue charcoal hidden mask
KbkxEmEmddSS blue charcoal hidden mask brindle or fawnt
KbKbEmEddSS blue charcoal hidden mask lack of mask
KbkxEmEddSS blue charcoal hidden mask, brindle or fawn, lack of mask
KbKbEEddSS blue charcoal none
KbkxEEddSS blue charcoal brindle or fawn
kxkxEEDDSS brindle or fawn black none
kxkxEEDdSS brindle or fawn black dilute
kxkxEmEmDDSS brindle or fawn with mask black none
kxkxEmEDDSS brindle or fawn with mask black lack of mask
kxkxEmEmDdSS brindle or fawn with mask black dilute
kxkxEmEDdSS brindle or fawn with mask black lack of mask, dilute
kxkxEEddSS diluted brindle or fawn charcoal none
kxkxEmEmddSS diluted brindle or fawn with mask charcoal none
kxkxEmEddSS diluted brindle or fawn with mask charcoal lack of mask
PRICING
Any single tested allele - $55.00*
Each additional allele - $20.00*
*subject to HST for Canadian residents only.

Note: Mask gene (Em) is considered as an individual test, separate from other alleles in E locus.

To help you to calculate the proper amount, please review the following example:
Testing for 3 alleles - E, B, and D - should be calculated as $55 + $20 + $20 = $95.00.
The price will be automaticaly calculated during the process of ordering a sample collection kit.
SAMPLES
HealthGene requires a blood sample (at least 1ml of whole blood in a purple top EDTA tube) for testing 3 or more alleles. If testing less than 3, a sample collection kit (cheek swab collection) can be ordered online following the link: Order A Sample Collection Kit.
TEST RESULTS
Test results are usually available in 10 Business Days from the moment the samples arrive at the laboratory. Test results can be reported by e-mail, fax, or by phone.
CERTIFICATE OF RESULT
HealthGene will provide a certificate for each test result.