Canine Coat and Nose Color Testing

Cavalier King Charles Spaniel occur in four colors: black-and-tan, tricolor, ruby, and blenheim. Two genes are responsible for these colors.
All Cavaliers are a^t/a^t but black-and-tan is only visible in the presence of at least one E allele. The e/e genotype causes ruby or blenheim because it masks the a^t/a^t genotype. Cavaliers with an e/e genotype and no white markings are called ruby, but those with white markings are called blenheim instead of ruby-and-white.
Cavaliers that are black-and-tan with white markings are tricolor. White markings are inherited as an automosomal recessive so dogs with white must be s^p/s^p.
Cavaliers in most countries are not brown. If pertinent, they could be tested for the b alleles at additional charge of $20.00.